Gene silencing by RNA Interference in the white rot fungus Phanerochaete chrysosporium.
Identifieur interne : 000688 ( Main/Exploration ); précédent : 000687; suivant : 000689Gene silencing by RNA Interference in the white rot fungus Phanerochaete chrysosporium.
Auteurs : Avi Matityahu [Israël] ; Yitzhak Hadar ; Carlos G. Dosoretz ; Paula A. BelinkySource :
- Applied and environmental microbiology [ 1098-5336 ] ; 2008.
Descripteurs français
- KwdFr :
- ARN fongique (génétique), ARN messager (génétique), Extinction de l'expression des gènes (MeSH), Gènes fongiques (MeSH), Interférence par ARN (MeSH), Phanerochaete (enzymologie), Phanerochaete (génétique), Plasmides (MeSH), Protéines fongiques (génétique), Réaction de polymérisation en chaîne (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Superoxide dismutase (génétique), Vecteurs génétiques (MeSH).
- MESH :
- enzymologie : Phanerochaete.
- génétique : ARN fongique, ARN messager, Phanerochaete, Protéines fongiques, Superoxide dismutase.
- Extinction de l'expression des gènes, Gènes fongiques, Interférence par ARN, Plasmides, Réaction de polymérisation en chaîne, Régulation de l'expression des gènes fongiques, Vecteurs génétiques.
English descriptors
- KwdEn :
- Fungal Proteins (genetics), Gene Expression Regulation, Fungal (MeSH), Gene Silencing (MeSH), Genes, Fungal (MeSH), Genetic Vectors (MeSH), Phanerochaete (enzymology), Phanerochaete (genetics), Plasmids (MeSH), Polymerase Chain Reaction (MeSH), RNA Interference (MeSH), RNA, Fungal (genetics), RNA, Messenger (genetics), Superoxide Dismutase (genetics).
- MESH :
- chemical , genetics : Fungal Proteins, RNA, Fungal, RNA, Messenger, Superoxide Dismutase.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- Gene Expression Regulation, Fungal, Gene Silencing, Genes, Fungal, Genetic Vectors, Plasmids, Polymerase Chain Reaction, RNA Interference.
Abstract
The effectiveness of RNA interference (RNAi) is demonstrated in the lignin-degrading fungus Phanerochaete chrysosporium. The manganese-containing superoxide dismutase gene (MnSOD1) was used as the target for RNAi. The plasmid constructed for gene silencing contained a transcriptional unit for hairpin RNA expression. Significantly lower MnSOD expression at both the mRNA and protein activity levels was detected in RNAi transformants. Furthermore, even though P. chrysosporium possesses three copies of the MnSOD gene, this RNAi construct was sufficient to decrease the enzymatic activity by as much as 70% relative to control levels. Implementation of the RNAi technique in P. chrysosporium provides an alternative genetic tool for studies of gene function, particularly of essential genes or gene families.
DOI: 10.1128/AEM.02433-07
PubMed: 18606804
PubMed Central: PMC2546648
Affiliations:
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Dosoretz</name></author><author><name sortKey="Belinky, Paula A" sort="Belinky, Paula A" uniqKey="Belinky P" first="Paula A" last="Belinky">Paula A. 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Dosoretz</name></author><author><name sortKey="Belinky, Paula A" sort="Belinky, Paula A" uniqKey="Belinky P" first="Paula A" last="Belinky">Paula A. Belinky</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="eISSN">1098-5336</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fungal Proteins (genetics)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Gene Silencing (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Plasmids (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>RNA Interference (MeSH)</term><term>RNA, Fungal (genetics)</term><term>RNA, Messenger (genetics)</term><term>Superoxide Dismutase (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN fongique (génétique)</term><term>ARN messager (génétique)</term><term>Extinction de l'expression des gènes (MeSH)</term><term>Gènes fongiques (MeSH)</term><term>Interférence par ARN (MeSH)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Plasmides (MeSH)</term><term>Protéines fongiques (génétique)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Superoxide dismutase (génétique)</term><term>Vecteurs génétiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>RNA, Fungal</term><term>RNA, Messenger</term><term>Superoxide Dismutase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN fongique</term><term>ARN messager</term><term>Phanerochaete</term><term>Protéines fongiques</term><term>Superoxide dismutase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term><term>Gene Silencing</term><term>Genes, Fungal</term><term>Genetic Vectors</term><term>Plasmids</term><term>Polymerase Chain Reaction</term><term>RNA Interference</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Extinction de l'expression des gènes</term><term>Gènes fongiques</term><term>Interférence par ARN</term><term>Plasmides</term><term>Réaction de polymérisation en chaîne</term><term>Régulation de l'expression des gènes fongiques</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effectiveness of RNA interference (RNAi) is demonstrated in the lignin-degrading fungus Phanerochaete chrysosporium. The manganese-containing superoxide dismutase gene (MnSOD1) was used as the target for RNAi. The plasmid constructed for gene silencing contained a transcriptional unit for hairpin RNA expression. Significantly lower MnSOD expression at both the mRNA and protein activity levels was detected in RNAi transformants. Furthermore, even though P. chrysosporium possesses three copies of the MnSOD gene, this RNAi construct was sufficient to decrease the enzymatic activity by as much as 70% relative to control levels. Implementation of the RNAi technique in P. chrysosporium provides an alternative genetic tool for studies of gene function, particularly of essential genes or gene families.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18606804</PMID><DateCompleted><Year>2008</Year><Month>09</Month><Day>30</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-5336</ISSN><JournalIssue CitedMedium="Internet"><Volume>74</Volume><Issue>17</Issue><PubDate><Year>2008</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Gene silencing by RNA Interference in the white rot fungus Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>5359-65</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/AEM.02433-07</ELocationID><Abstract><AbstractText>The effectiveness of RNA interference (RNAi) is demonstrated in the lignin-degrading fungus Phanerochaete chrysosporium. The manganese-containing superoxide dismutase gene (MnSOD1) was used as the target for RNAi. The plasmid constructed for gene silencing contained a transcriptional unit for hairpin RNA expression. Significantly lower MnSOD expression at both the mRNA and protein activity levels was detected in RNAi transformants. Furthermore, even though P. chrysosporium possesses three copies of the MnSOD gene, this RNAi construct was sufficient to decrease the enzymatic activity by as much as 70% relative to control levels. 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IdType="pubmed">3315461</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Israël</li></country></list><tree><noCountry><name sortKey="Belinky, Paula A" sort="Belinky, Paula A" uniqKey="Belinky P" first="Paula A" last="Belinky">Paula A. Belinky</name><name sortKey="Dosoretz, Carlos G" sort="Dosoretz, Carlos G" uniqKey="Dosoretz C" first="Carlos G" last="Dosoretz">Carlos G. Dosoretz</name><name sortKey="Hadar, Yitzhak" sort="Hadar, Yitzhak" uniqKey="Hadar Y" first="Yitzhak" last="Hadar">Yitzhak Hadar</name></noCountry><country name="Israël"><noRegion><name sortKey="Matityahu, Avi" sort="Matityahu, Avi" uniqKey="Matityahu A" first="Avi" last="Matityahu">Avi Matityahu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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